J Cell
Physiol. 2004 Mar;198(3):479-80. |
L'exposition à un champ électromagnétique 900 MHZ
incite un déséquilibre entre les signaux pro apoptotique et de pro survie dans
des cellules de leucémie lymphoblastique.
Exposure
to 900 MHz electromagnetic field induces an unbalance between pro-apoptotic and
pro-survival signals in T-lymphoblastoid leukemia CCRF-CEM cells.
Marinelli F, La Sala D, Cicciotti G, Cattini L, Trimarchi C, Putti S,
Zamparelli A, Giuliani L, Tomassetti G, Cinti C.
Institute for Organ Transplantation
and Immunocytology, ITOI-CNR, Bologna unit, c/o IOR, Bologna, Italy.
The original article to which this Erratum was published in J. Cell. Physiol.
198:324-332, 2004It has been recently established that low-frequency
electromagnetic field (EMFs) exposure induces biological changes and could be
associated with increased incidence of cancer, while the issue remains
unresolved as to whether high-frequency EMFs can have hazardous effect on
health. Epidemiological studies on association between childhood cancers,
particularly leukemia and brain cancer, and exposure to low- and high-frequency
EMF suggested an etiological role of EMFs in inducing adverse health effects.
To investigate whether exposure to high-frequency EMFs could affect in vitro
cell survival, we cultured acute T-lymphoblastoid leukemia cells (CCRF-CEM) in
the presence of unmodulated 900 MHz EMF, generated by a transverse
electromagnetic (TEM) cell, at various exposure times. We evaluated the effects
of high-frequency EMF on cell growth rate and apoptosis induction, by cell
viability (MTT) test, FACS analysis and DNA ladder, and we investigated
pro-apoptotic and pro-survival signaling pathways possibly involved as a
function of exposure time by Western blot analysis. At short exposure times
(2-12 h), unmodulated 900 MHz EMF induced DNA breaks and early activation of
both p53-dependent and -independent apoptotic pathways while longer continuous
exposure (24-48 h) determined silencing of pro-apoptotic signals and activation
of genes involved in both intracellular (Bcl-2) and extracellular (Ras and
Akt1) pro-survival signaling. Overall our results indicate that exposure to 900
MHz continuous wave, after inducing an early self-defense response triggered by
DNA damage, could confer to the survivor CCRF-CEM cells a further advantage to
survive and proliferate. J. Cell. Physiol. 198: 324-332, 2004. Copyright 2003
Wiley-Liss, Inc.