Bioelectromagnetics.
2004 Feb; 25(2): 118-26. |
Un bas champ électromagnétique (50 Hz) incite la
différentiation sur des kératinocytes primaires humains.
Low
electromagnetic field (50 Hz) induces differentiation on primary human oral
keratinocytes (HOK).
Manni V, Lisi A, Rieti S, Serafino A, Ledda M, Giuliani L, Sacco D,
D'Emilia E, Grimaldi S.
Istituto di Neurobiologia e
Medicina Molecolare (INeMM), CNR, Rome, Italy.
This work concerns the effect of low frequency electromagnetic fields (ELF) on
biochemical properties of human oral keratinocytes (HOK). Cells exposed to a 2
mT, 50 Hz, magnetic field, showed by scanning electron microscopy (SEM)
modification in shape and morphology; these modifications were also associated
with different actin distribution, revealed by phalloidin fluorescence
analysis. Moreover, exposed cells had a smaller clonogenic capacity, and
decreased cellular growth. Indirect immunofluorescence with fluorescent
antibodies against involucrin and beta-catenin, both differentiation and adhesion
markers, revealed an increase in involucrin and beta-catenin expression. The
advance in differentiation was confirmed by a decrease of expression of
epidermal growth factor (EGF) receptor in exposed cells, supporting the idea
that exposure to electromagnetic field carries keratinocytes to higher
differentiation level. These observations support the hypothesis that 50 Hz
electromagnetic fields may modify cell morphology and interfere in
differentiation and cellular adhesion of normal keratinocytes. Bioelectromagnetics
25:118-126, 2004. Copyright 2004 Wiley-Liss, Inc.