Int J Radiat Biol. 2004 Jan;80(1):11-20. |
Mesure de dégâts d'ADN après exposition aiguë à une
onde pulsée micro-onde de 2450 MHZ dans cellules de cerveau de rat par deux
méthodes de dosage.
Measurement
of DNA damage after acute exposure to pulsed-wave 2450 MHz microwaves in rat
brain cells by two alkaline comet assay methods.
Lagroye I, Anane R, Wettring BA, Moros EG, Straube WL, Laregina M, Niehoff
M, Pickard WF, Baty J, Roti JL.
PIOM/EPHE Bioelectromagnetics Laboratory ENSCPB 16 Avenue Pey Berland F-33607
Pessac France.
Purpose: To investigate the effect of 2450 MHz pulsed-wave microwaves on the
induction of DNA damage in brain cells of exposed rats and to discover whether
proteinase K is needed to detect DNA damage in the brain cells of rats exposed
to 2450 MHz microwaves. Materials and methods: Sprague-Dawley rats were exposed
to 2450 MHz pulsed-wave microwaves and sacrificed 4 h after a 2-h exposure.
Rats irradiated whole-body with 1 Gy (137)Cs were included as positive
controls. DNA damage was assayed by two variants of the alkaline comet assay on
separate aliquots of the same cell preparation. Results: Significant DNA damage
was observed in the rat brain cells of rats exposed to gamma-rays using both
versions of the alkaline comet assay independent of the presence or absence of
proteinase K. However, neither version of the assay could detect any difference
in comet length and/or normalized comet moment between sham- and 2450 MHz
pulsed-wave microwave-exposed rats, regardless of the inclusion or omission of
proteinase K in the comet assay. Conclusions: No DNA damage in brain cells was
detected following exposure of rats to 2450 MHz microwaves pulsed-wave at a
specific absorption rate of 1.2 W kg(-1) regardless of whether or not
proteinase K was included in the assay. Thus, the results support the
conclusion that low-level 2450 MHz pulsed-wave microwave exposures do not
induce DNA damage detectable by the alkaline comet assay.