Bioelectromagnetics.
2004 Feb; 25(2): 127-33. |
L'apoptose provoquée par la radiation ultra-violet est
augmentée par la radiation de
radiofréquence modulée en amplitude dans des cellules de levure.
Apoptosis
induced by ultraviolet radiation is enhanced by amplitude modulated
radiofrequency radiation in mutant yeast cells.
Markkanen A, Penttinen P, Naarala J, Pelkonen J, Sihvonen AP, Juutilainen J.
Department of Environmental Sciences, University of Kuopio, Kuopio, Finland.
The aim of this study was to investigate whether radiofrequency (RF)
electromagnetic field (EMF) exposure affects cell death processes of yeast
cells. Saccharomyces cerevisiae yeast cells of the strains KFy417 (wild-type)
and KFy437 (cdc48-mutant) were exposed to 900 or 872 MHz RF fields, with or
without exposure to ultraviolet (UV) radiation, and incubated simultaneously
with elevated temperature (+37 degrees C) to induce apoptosis in the
cdc48-mutated strain. The RF exposure was carried out in a special waveguide
exposure chamber where the temperature of the cell cultures can be precisely
controlled. Apoptosis was analyzed using the annexin V-FITC method utilizing
flow cytometry. Amplitude modulated (217 pulses per second) RF exposure
significantly enhanced UV induced apoptosis in cdc48-mutated cells, but no
effect was observed in cells exposed to unmodulated fields at identical
time-average specfic absorption rates (SAR, 0.4 or 3.0 W/kg). The findings
suggest that amplitude modulated RF fields, together with known damaging
agents, can affect the cell death process in mutated yeast cells.
Bioelectromagnetics 25:127-133, 2004. Copyright 2004 Wiley-Liss, Inc.